ZFIN Image: Bhuiyan et al., 2016, Fig. S4

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Figure Caption

Fig. S4

(A) Relative gene expression of IL-8 and Lta4H in wild-type A. baumannii (ATCC 17978) and ΔgacS-infected zebrafish embryos at 1 and 12 hpi. Compared with uninfected embryos, mRNA for IL-8 and Lta4H was increased, but there was no significant difference between wild-type A. baumannii and ΔgacS-infected embryos. Experiments were performed at least in triplicate with three biological replicates (mean ± SEM). (B) Neutrophil clustering was not seen for an A. baumannii mutant involving another GacS-regulated gene (csuD) in a somatic muscle (red rectangle) (i) or otic vesicle (red circle) (ii) infection at 48 hpi. (C) Neutrophil chemotaxis was seen, but dwelling was not observed for heat-killed A. baumannii ΔpaaA (HK ΔpaaA) infection at 48 hpi. (Magnification: B and C, 10×.)

Acknowledgments

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