ZFIN Image: Monteiro et al., 2016, Fig. S4

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Figure Caption

Fig. S4

Inhibition of Vegf (kdr+kdrl^MO), Wnt (Wnt16^MO) or BMP (BMP4^MO) signalling pathways by morpholino oligonucleotides. All in situ hybridisation experiments were performed at 22hpf except for the runx1 probe, performed at 26hpf. (A) Loss of Vegf signalling upon morpholino knockdown of the Vegf receptors kdrl and kdrl (Bahary et al, 2007) results in decreased expression of tgfb1a and tgfb1b in the dorsal aorta (DA). Runx1 expression in the DA was used as a positive control for the experiment. (B) Loss of Wnt signalling or BMP signalling by morpholino knockdown of Wnt16 (Clemens et al, 2011) or BMP4 (Chocron et al, 2007) showed no effect of expression of TGFβ ligands or receptor. Runx1 expression in the DA was used as a positive control for the experiment. Numbers of embryos analysed are shown in each panel as number of affected embryos/total observed.

Acknowledgments

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Reprinted from Developmental Cell, 38(4), Monteiro, R., Pinheiro, P., Joseph, N., Peterkin, T., Koth, J., Repapi, E., Bonkhofer, F., Kirmizitas, A., Patient, R., Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells, 358-70, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell