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Fig. S2
Effects of treating zebrafish embryos with the indicated doses of cortisol from fertilization up to 5 days post-fertilization (dpf) on (A) average heart rate at 3 dpf (20 larvae per sample; p = 1.54 x 10-12 by one-way ANOVA; * indicates that the difference from untreated controls is statistically significant by post-hoc Tukey’s test); and (B) reactive oxygen species levels in tail muscle, measured by backgroundcorrected DCF fluorescence at 5 dpf (5 larvae imaged per sample, in two biological replicates; p = 5.43 x 10-6 by one-way ANOVA; * indicates that the difference from untreated controls is statistically significant by post-hoc Tukey’s test). Error bars represent SEM. Example images are shown beneath the graph.