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Fig. 8
Behavioral evaluations in 5 dpf morphants showing the reduction of swimming ability.
Both total distance and average velocity in swimming behavior were decreased in ddc morphants. (A) Swimming routes of a 5 dpf larva with injection of none, 1x injection buffer, 5 ng tMO1,100 pg mRNA and 5ng tMO1 and 100 pg ddc mRNA mixture to rescue, respectively. The video was recorded for 25 min. (B) Statistical analysis showed total distance of trajectories. The ddc morphants revealed decreased total distance of swimming compared with different controls. However, co-injection of 100 pg full length ddc mRNA can significantly attenuated the reduction of total swimming distance. (C) Statistical analysis for swimming velocity among different controls and ddc morphants, and rescued larvae also revealed the same results. The bars represent changes in different treatment of 5 dpf larvae in three independent experiments. n=31 for each treated larvae. Results were expressed as mean±SD. *p<0.05 compared to control larvae.