Fig. 5
Zf12-LO is required for normal eye development. Sections of zf embryos were prepared and stained with Hematoxylin/Eosin. Morphological comparison of zf12-LO morphants (treated with MO-2) and controls (treated with MO-2 mis) was carried out with light microscopy at two dpf. Injection of MO-2 results in developmental eye abnormalities including loss of retinal layered structures and lens crystalline. RPE; retinal pigmented epithelium, RCL; rods and cones layer, OPL; outer plexiform layer, BCL; bipolar cell layer, ACL; amacrine cell layer, IPL; inner plexiform layer, GCL; ganglion cell layer. Similar results were obtained with zf12-LO morphants treated with MO-1.