Fig. 3
Frequencies of cardiac and trunk defects in Smyd3 morphants treated with different concentration of morpholinos, and with or without pre-injection with smyd3_tv1 or smyd3_tv2 mRNA.
(A) Frequencies of heart defects in embryos injected with 1.5 or 3.0 ng of Smyd3-MO, 3.0 ng of Smyd3-mis-MO, 3.0 ng of Smyd3-SB-MO, 3.0 ng of Smyd3-MO in combination with smyd3_tv1 or smyd3_tv2 mRNA at 48 hpf. The histogram shows percentages of embryos with Normal (open box), Grade1 (light gray box), Grade2 (dark gray box), Grade3 (hatched box) or dead embryos (closed box). (B) Frequencies of curved trunk in embryos injected with 1.5 or 3.0 ng of Smyd3-MO, 3.0 ng of Smyd3-mis-MO, 3.0 ng of Smyd3-SB-MO, 3.0 ng of Smyd3-MO in combination with smyd3_tv1 or smyd3_tv2 mRNA at 48 hpf. The histogram shows percentages of embryos with Normal (open box), curved trunk (gray box), or dead embryos (closed box). Error bars represent the SEM. N is the total number of injected animals.