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Fig. 3
ptenb MOs delays epibolic progression.
Knockdown of ptenb by MOs caused delay in epibolic movement in a dose-dependent manner. (A) Embryos were injected with standard MO (StdMO), tMO1, 5-bp mismatched tMO1 (mis-tMO1) and tMO2 at designated amount, incubated to 10 hpf and the percentages of embryos at each epiboly stage are shown. (B) Embryos were injected with 15 ng of StdMO or tMO1 in the absence or presence of ptenb mRNAs (50 pg), incubated and their degrees of epiboly progression are shown. Each treatment was repeated at least 6 times.
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