ZFIN Image: Xu et al., 2016, Fig. S4

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Figure Caption

Fig. S4

LPC-G2A Pathway Is Required for Microglial Precursors Entering the Brain, related to Figure 4

(A) AO staining showed that no excess apoptotic cells were observed in the brain injected with ATPγS or LPC, although the apoptotic cells could be readily observed in the brain of 3 dpf WT.

(B) Dorsal view images of the midbrain of Tg(mpeg1:loxP-DsRedx-loxP-eGFP) control and gpr132a morphants (MO). DsRedx+ cells represent microglia. Dashed lines indicate the midbrain.

(C) Quantification of the microglia number in the midbrain of Tg(mpeg1:loxP-DsRedx-loxPeGFP) control and gpr132a morphants (MO). n=8 for WT control and n=10 for MO. Error bars represent mean SEM.

(D) Examination of gpr132a and gpr132b expression in macrophage and whole fish body by RTPCR at 2.5-3 dpf.

(E) gpr132a MO efficiently blocked the expression of pCS2-gpr132a-ATG-DsRedx reporter construct.

(F) gpr132b MO efficiently blocked the expression of pCS2-gpr132b-ATG-DsRedx reporter construct.

Figure Data

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Acknowledgments

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Reprinted from Developmental Cell, 38(2), Xu, J., Wang, T., Wu, Y., Jin, W., Wen, Z., Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine, 214-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell