ZFIN Image: Shang et al., 2011, Fig. 6

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Figure Caption

Fig. 6

Knockdown of p53 suppresses the DNA damage-induced expression of zebrafish rrm2b gene. Embryos at one-cell stage were injected with 1.5 ng of p53 MO or the standard control (CK) MO. Developing embryos at 24 hpf were treated with 2 µM of CPT or MMS for 6 h and collected for total RNA extraction. Real-time PCR was then performed to determine the expression of p53R2. The ratio of p53R2 to β-actin in untreated embryos was set to 1 in each group, and all treated embryos were normalized relative to this value. Data are expressed as means ± SD from three independent experiments. * indicated p < 0.05 versus the control.

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Acknowledgments

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Reprinted from Gene, 475(1), Shang, H., Li, Q., Feng, G., and Cui, Z., Molecular analysis and functions of p53R2 in zebrafish, 30-38, Copyright (2011) with permission from Elsevier. Full text @ Gene