IMAGE

Fig. 2

ID
ZDB-IMAGE-160823-30
Source
Figures for Shimoda et al., 2005
Image
Figure Caption

Fig. 2

Suppression of Dnmt1 or Dnmt7 activity affects ntl methylation. A: Methylation status of the ntl CpG island was analyzed 48 hr after the injection of morpholino oligonucleotide (MO) against each dnmt. mm-dnmt1 and mm-dnmt7 are negative-control MOs carrying mismatch nucleotides in their original dnmt1-MO and dnmt7-MO sequences, respectively. Part of the ntl CpG island containing 16 CpG dinucleotides was PCR amplified and 10 independent clones were sequenced. Each column represents the mean ± standard error of methylated cytosines. B:ntl methylation level reduced by dnmt7 52UTR-MO was partially restored by coinjection of in vitro-synthesized dnmt7 mRNA (approximately 500 pg), which has no complementary sequence to the dnmt7 5′UTR-MO. Also, 200 pg of the same mRNA was unable to rescue the de novo methylation (data not shown). Because dnmt7 52 UTR-MO caused a neural death phenotype that at low doses is transient, we used less of the 52 UTR-MO (1.5 ng) than that of the original dnmt7-MO (2.0 ng). C: Methylation status of an ntl 32 exon region. An ntl 3′ region harboring 15 CpGs was amplified, and 10 independent clones were sequenced.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.