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Fig. 6
prep1.1 morphants lack the pharyngeal skeleton. Alcian blue staining of the head skeleton of 5 day-old prep1.1 morphants (C,D) reveals the complete lack of all pharyngeal cartilages, while those of the neurocranium are reduced. The wild-type phenotype can be substantially rescued by coinjection of prep1.1-MOb with a prep1.1 mRNA construct lacking the sequence complementary to MOb (E,F). In pbx4 morphants (G,H), the mandibular and hyoid cartilages are present, but are aberrant in shape and size and abnormally fused (arrowhead). Larvae are in lateral (A,C,E,G) and ventral (B,D,F,H), views with anterior to the left. cb, ceratobranchial; ch, ceratohyal; e, ethmoid plate; hs, hyosymplectic; me, Meckel′s cartilage; n, notochord; oa, occipital arch; pch, parachordal; pq, palatoquadrate; tr, trabeculae cranii.