ZFIN Image: Naylor et al., 2016, Fig. 7

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Figure Caption

Fig. 7

RA acts downstream of the BMP signalling pathway during kidney patterning.

(a) Whole-mount double in situ hybridization analysis for nephron segment markers slc4a4 (red) and slc12a3 (purple) in embryos at the 15-somites stage injected with bmp2b mRNA or treated with DM dihydrochloride, DEAB and DMSO (vehicle control) at the indicated stages. Embryos are shown as dorsal views with anterior to the left. (b) Embryos were treated as shown and stopped at the 15-somites stage for double in situ hybridization staining for the anterior pronephros marker slc4a4 and the distal pronephros marker slc12a3. All panels are dorsal views of flatmounted embryos. Scale bar, 200 µm.

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Acknowledgments

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