ZFIN Image: Bolar et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Suppression of SEC61A1 Leads to Pronephric Tubular Atrophy in Zebrafish

(A-C) Whole-mount immunostaining of 4 days post-fertilization (dpf) zebrafish larvae with anti-Na⁺/K⁺-ATPase alpha subunit monoclonal antibody (±6F) shows the overall anatomy of the pronephric ducts (pd) and pronephric tubules (pt), which become progressively convoluted in control larvae. Three levels of convolution were assessed: convoluted (normal) (A and A′), V-shaped (B and B′), and straight pronephric tubule (C and C′). The pronephric ducts are normal.

(D) Qualitative scoring of the tubular atrophy was performed in larvae batches injected with sec61al2 MO alone; MO and mutant (p.Thr185Ala) RNA or (p.Val67Gly) RNA; MO and wild-type (WT) capped-RNA; WT and mutant RNAs alone; and control.

(E) Results of Fisher’s exact test conducted between pairs of conditions. The significant p values (< 0.05) are highlighted in red.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Hum. Genet.