ZFIN Image: Rodriguez-Mari et al., 2011, Fig. 2

Image

Figure Caption

Fig. 2

Expression of brca2 in wild-type gonads.

(A,B) 26dpf transitioning stage ovaries and testes, respectively, express brca2 in the ooplasm of oocytes (arrow) and in male germ cells. (C,D) Expression of brca2 increases in 33dpf immature ovaries and testis, respectively. Arrows indicate examples of brca2-expressing cells. (E) In wild-type ovaries, brca2 transcript is highly expressed in the ooplasm of late stage IB oocytes (L-IB, arrow) and gradually becomes localized to a small portion of the ooplasm in stage III and IV oocytes (arrowheads). (F) Spermatocytes contain brca2 transcript but spermatids and sperm do not. (G) Stage III-IV oocytes accumulate brca2 transcript in a pole of the oocyte. (H) Comparison to the adjacent section probed for the animal pole marker pou5f1(oct4) reveals that brca2 transcript localizes to the animal pole in the same location as pou5f1(oct4) message (arrows in G and H). Abbreviations: II, III, IV, stages of oocyte development; L-IB, late stage IB oocyte (stages according to [49]); do, degenerating oocyte; GV, germinal vesicle; sc, spermatocyte; sp, sperm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.