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Fig. 6
The Toll-like signaling adaptor molecule Myd88 and antibiotics protect against trinitrobenzene sulfonic acid (TNBS) -induced mortality. A: Comparison of Myd88 morphant survival with that of control larvae exposed to TNBS at a concentration of 75 µg/ml for 48 hr. Survival is expressed as a percentage of the original cohort (n = 5, groups of 20 larvae). B: Time course comparison of survival between larvae co-treatment with broad-spectrum antibiotics and control larvae exposed TNBS at a concentration of 100 µg/ml (n = 4, groups of 20 larvae). C: Quantitative polymerase chain reaction analysis of pro-inflammatory cytokine transcription in larvae exposed to TNBS with or without antibiotic administration (n = 5, pools of 20 larvae). All error bars represent at 95% confidence interval of the mean, P values calculated by t-test. *P < 0.05, **P < 0.01, ***P < 0.001.