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Fig. 7 Optokinetic response test to reveal visual impairments. Semi-quantitative scoring of the optokinetic response (OKR) test at 100 hpf and 7 dpf for control, KD and rescue larvae. Data show a significant delay for D3bMO when compared to control and D1D2MO larvae at 100 hpf and compared to all groups at 7 dpf (p < 0.001 and p < 0.05 respectively, Chi-square statistics). Co-injection with hD3 mRNA partially rescues the phenotype. SCMO: control group, D1D2MO: KD of D1+D2; D3bMO: KD of D3b, D3bMO + hD3 RNA: rescue group; Full OKR: normal optokinetic response with the eyes following the movement of the stripes and returning to their original position; Partial OKR: the eyes follow the movement of the stripes but do not return to their original position after reaching the border of the visual field; no OKR: fish do not respond to the moving stripes.
Reprinted from Molecular and Cellular Endocrinology, 424, Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M., Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function, 81-93, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.