ZFIN Image: McCarthy et al., 2016, Fig. 1

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Fig. 1

The postchordal neurocranium requires Fgf signaling. (A-E) Wholemounted zebrafish neurocrania with the viscerocrania removed at 5 days post-fertilization. Anterior is to the left. (A) Wildtype, showing the prechordal versus postchordal regions of the neurocranium demarcated by a dashed line, (B) fgf8a mutant, (C) and fgf3 mutant embryos display normal neurocrania. (D) Variable neurocranial defects occur in 63% (n=5/8) of fgf8a^-/-; fgf3^+/- embryos (arrowheads). (E) The postchordal neurocranium in fgf8a^-/-; fgf3^-/- embryos is almost completely absent in 83% (n=5/6) of embryos, including the parachordals, anterior and posterior basicapsular commissure and the lateral commissures (arrowhead). abc-anterior basicapsular commissure, lc-lateral commissure, n-notochord, oc-occipital arch, pbc-posterior basicapsular commissure, pc-parachordals, prech.=prechordal neurocranium, postch.=postchordal neurocranium. Scale bar=20 µm.

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Acknowledgments

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Reprinted from Developmental Biology, 415(2), McCarthy, N., Sidik, A., Bertrand, J.Y., Eberhart, J.K., An Fgf-Shh signaling hierarchy regulates early specification of the zebrafish skull, 261-77, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.