ZFIN Image: He et al., 2016, Fig. 5

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Figure Caption

Fig. 5

Loss of nf1 suppresses apoptosis and enhances proliferation of tumor cells in MYCN-driven neuroblastoma.

Immunohistochemical analysis of sagittal sections through tumors in the IRG of nf1a+/+;nf1b+/+;MYCN;GFP (A,D), nf1a+/-;nf1b+/+;MYCN;GFP (B,E) and nf1a-/-;nf1b+/+;MYCN;GFP (C,F) fish at the age of 6 weeks, using antibodies against cleaved caspase-3 (CC3; A-C) and proliferating cell nuclear antigen (PCNA; D-F).Dotted lines indicate tumor boundaries. Quantification of CC3-positive (G) and PCNA-positive (H) cells. Values are means + s.e.m. per section (three nf1a+/+;nf1b+/+;MYCN;GFP, five nf1a+/-;nf1b+/+;MYCN;GFP and four nf1a-/-;nf1b+/+;MYCN;GFP tumors). *p<0.05, **p<0.01, ***p<0.001 by two-tailed unpaired t-test.

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Acknowledgments

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