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Fig. S3
Endothelial cell polarization in DA and PCV.
(a) 3D-rendered confocal stack images of the trunk region of a 30 hpf Tg(kdrl:NLS-EGFP);Tg(fli1a:B4GALT1-mCherry) embryo. (b, c) Confocal time-lapse images of the ECs in the white (b) and red (c) boxes in (a). Surface rendered images are displayed below. White arrowheads point to polarized ECs, yellow arrowheads to non-polarized ECs. Red asterisk indicates the time when vigorous blood circulation starts. Yellow asterisk indicates the time when ECs are polarized. White dashed arrows indicate the direction of blood flow. (d) 3D-rendered confocal stack images of ECs undergoing cell division in the trunk region of a 36 hpf Tg(kdrl:NLS-EGFP);Tg(fli1a:B4GALT1- mCherry) embryo. (e) Time-lapse image of white box in (d). Surface rendered images are displayed below. White asterisks indicate ECs under cell division. White arrowheads point to the Golgi apparatus. White arrows indicate the direction of blood flow. Time (hours:mins) is shown in the top right corner of the images. (f) Schematic representations showing EC polarization after the onset of blood flow (i) and after cell division under vigorous blood flow (ii). (g, h) 3D-rendered confocal stack images of the trunk region of a 36 hpf Tg(kdrl:NLS-EGFP);Tg(fli1a:B4GALT1-mCherry) embryo. (h) Time-lapse images of the white box in (g). Surface rendered images are displayed below. White arrowheads and yellow arrowheads point to ECs undergoing cell division. Time (hours:mins) is shown in the top right corner of the images. Anterior to the left, dorsal to the top. Scale bars, 20 µm (a,d and g), 7 µm (b, c, e and h). DA, dorsal aorta; PCV, posterior cardinal vein.