Fig. 5
PGP midline convergence phenotypes in ntl-/- embryos. ((A)-(E)) Whole-mount in situ hybrydization of wt1a and myoD was stained blue and orange, respectively in 12 (A), 24 (B) and 48 hpf (C) ntl-/- embryos. The scale bar indicates 100 µm; ((A)-(C)) are at the same magnification. All images are dorsal view anterior to the top. (D) Quantification of convergence morphogenetic movements in ntl-/- embryos and comparison with WT. The inter distance of PGP was measured at 11 (n=20, P=0.68), 12 (n=17, P=0.27), 24 (n=23,), 36 (n=29), 48 (n=20) hpf. The inter distance of PGP is significant difference between ntl-/- and WT at 24, 36 and 48 hpf (***P<0.0005). Error bars represent SD.
Reprinted from Developmental Biology, 384(2), Huang, C.J., Wilson, V., Pennings, S., MacRae, C.A., and Mullins, J., Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis, 290-300, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.