ZFIN Image: Soulika et al., 2016, Fig. 1

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Figure Caption

Fig. 1

tg(fgf8a.dr17:gfp) expression in developing taste bud cells. In all figures unless otherwise mentioned images are ventral views, anterior to the left. (A) Dissection scope. (B,C) 10µm spinning-disk images, live larvae. In A, mouth epithelium (lips and wall) is within the blue dotted circle. Time-lapse imaging focused on anterior mouth epithelium (green dotted rectangle). White arrowheads indicate anterior-medial mouth where a taste bud forms (A,B) and a cell co-expressing tg(fgf8a.dr17:gfp) and tg(tph1b:mCherry) (C, red dots), respectively. (D-K) 2µm confocal sections. In E-K, each column shows merge, taste bud marker (red) and tg(fgf8a.dr17:gfp) expression (green). In D,H,I, orange arrowheads indicate tg(fgf8a.dr17:gfp) cells that co-express Calb2 /plcb2. Scale bars: 10µm.

Acknowledgments

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