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Fig. 4
Generation of adam12:EGFP transgenic zebrafish. (A) The colored box is the DNA construction for generation of the adam12:EGFP transgenic zebrafish. A 12 979-bp upstream region of adam12 is connected to egfp (green) across an intron (gray). A SV40-polyA sequence (purple) is added to the tail of the EGFP. The adam12:EGFP cassette is carried by the Tol2 terminal sequences (orange). The waveforms indicate the comparison of the 12 979-bp with corresponding region of medaka, mouse and human. The 12 979-bp contains the conserved region that presumed to the regulatory elements for ADAM12 gene (blue dashed-squares). (B-D) Micrographs of fluorescence imaging of the adam12:EGFP transgenic zebrafish at 16-hpf (B), 24-hpf (C), and 3-dpf (D). B′ shows a dorsal view of the posterior LPM; C′ shows a dorsal view of the heart primordium; D′ shows a dorsal view of the cephalic nerve; and D′′ shows a ventral view of the heart. HP, heart primordium; HT, heart; ICM, intermediate cell mass; LPM, lateral plate mesoderm; MB, midbrain; PBI, posterior blood island; VA, ventral aorta. Scale bar: 200 µm.