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Figure Caption
Fig. 8
Detection of endogenous hydrogen sulfide by L1 in zebrafish embryos in vivo.All embryos at 27 h postfertilization. Zebrafish embryos were imaged after 2 h pretreatment with control (deionized water, left column (a,c,e)) or 100 µM AOAA pretreatment (right column (b,d,f)), followed by incubation of embryos with 0.01% DMSO control (a,b), 5 µM L1(c,d), 25 µM L1 (e,f) for 30 min. (g) The measurement of the mean fluorescence intensity of the whole embryonic body as region of interest (ROI). Arrow: yolk; arrowheads: brain; bracket: trunk. Scale bar = 400 µm.
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