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Fig. S4
Formalin-fixation causes alterations in chlorine distribution. Alterations in Cl distribution are seen when the sample is formalin-fixed (A-C) as an additional sample preparation step prior to flash freezing cartilage, in comparison with no formalin-fixation (D-F). The quantitative comparison is given in (G). ** indicates significantly increased Cl in samples formalin-fixed prior to cryo-fixation, p < 0.01, Student’s t-test, 95% confidence.
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