ZFIN Image: Ozaki et al., 2011, Fig. 4

Image

Figure Caption

Fig. 4

Expression of Cyclin B3 in the zebrafish testis by immunohistochemistry. (A and C) The section of a testis immunostained with antibodies against Cyclin B3. Allows and arrowheads indicate type A spermatogonia and type B spermatogonia, respectively. Green signals indicate Cyclin B3 and red staining indicates nuclei. S, sperm. (B and D) Immunostained with Sycp3 in the sequential section to (A) and (C). Green signals indicate Sycp3 and red staining indicates nuclei. S, sperm. Panels (C) and (D) are at higher magnification of the white dotted area in (A) and (B), respectively. (E-G) Immunostaining for Cyclin B3 in the 4-cell clone (E), the 8-cell clone (F), and the 16-cell clone (G). More than 10 clones of each were observed in two fish. White dots indicate surrounding Sertoli cells of the cyst. Bars, 20 µm (A, B, and E-G), 10 µm (C and D).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 11(5-6), Ozaki, Y., Saito, K., Shinya, M., Kawasaki, T., and Sakai, N., Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish, 309-315, Copyright (2011) with permission from Elsevier. Full text @ Gene Expr. Patterns