ZFIN Image: Nepal et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Expression pattern revealed by in situ hybridization of mature miR-9 and pri-miRNA transcripts during prim6 stage. (A) Expression pattern of mature miR-9 is mostly restricted to telencephalon. Left panels represent lateral views and right panels represent dorsal views. (B-I) Expression pattern of miR-9 pri-miRNA transcripts is also restricted to telencephalon with differences in the spatiotemporal details among different pri-miRNAs, such as: miR-9-2 (developing retina, white asterisk; Figure 2C), miR-9-4 (posterior brain regions, white arrowhead; Figure 2E) and miR-9-6 (hypothalamus, white arrow; Figure 2H). (J) Amplified region of 5′ RACE PCR product (corresponding to probe-1) of miR-9-5 maps exactly to the alternative intronic CAGE tags. (K) Unique discrete amplification band obtained from 5′ RACE PCR products obtained for miR-9-5 (with intronic CAGE tags). Multiple non-specific bands and/or a smear were obtained for miR-9-4 that did not have alternative intronic CAGE tags.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.