ZFIN Image: Kent et al., 2016, Fig. S1

Image

Figure Caption

Fig. S1

Asymmetric epiboly is specific to UHRF1 overexpression.

(A) Progression of embryonic development in uninjected and embryos injected with 100 pg of mRNA encoding EGFP. Effective EGFP mRNA translation was determined by fluorescence. EGFP overexpression does not elicit developmental abnormalities. Scale bar = 500 micrometers. (B) Embryos injected with 100 pg mRNA encoding zebrafish WT-Uhrf1 and Uhrf1^S648A develop asymmetric epiboly with a similar incidence as those injected with the human homologs. (C) Kaplan-Meier survival curve of WT-UHRF1 overexpressing embryos. Significance was determined by the Log-rank Test (p < 0.0001). (D) Graph of 24 biological replicates of WT-UHRF1 mRNA injected embryos scored at 6 hpf plotted with the total number of embryos per clutch per treatment with indicated phenotype distribution.

Acknowledgments

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Reprinted from Developmental Biology, 412(1), Kent, B., Magnani, E., Walsh, M.J., Sadler, K.C., UHRF1 regulation of Dnmt1 is required for pre-gastrula zebrafish development, 99-113, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.