ZFIN Image: Stiff et al., 2016, Fig. S2

Image

Figure Caption

Fig. S2

Atr morphant zebrafish display morphological phenotypes typical of cilia dysfunction.

A) Live images of 48 hpf zebrafish embryos showing pericardiac edema upon Atr knockdown. Zebrafish were either left uninjected (NI) or control injected with a standard control MO (CTRL MO) or the translation blocking Atr MO.

B) Percentage of embryos developing pericardiac edema. Graph displays mean values ± SEM, n=79-85 embryos in 3 experiments. ** indicates p<0.01, One way ANOVA.

C) Treatment with the ATR inhibitor from tailbud stage causes pericardiac edema, too. Graph displays means ± SEM, n=55-60 embryos in 3 experiments. p=0.0025, Student’s t-test.

D) Live image of a clutch of 48 hpf embryos that had been injected with Atr MO at the one cell stage. Note the large number of embryos displaying an increased body curvature, which is an indicator of a cilia dysfunction.

E) Percentage of embryos with an abnormally curved body. Graph displays mean values ± SEM, n=79-85 embryos in 3 experiments. ** indicates p<0.01, One way ANOVA.

F) Chemical inhibition of Atr (Atri) robustly leads to curved embryos. Results show the mean values ± SEM, n=55-60 embryos in 3 experiments. p<0.0001, Student’s t-test.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.