Fig. 6, S1 Loss of Yap1 in MZamotl2a-/-;MZyap1-/- embryos supresses increased pLLP cell number resulting from loss of Amotl2a in MZamotl2a-/- embryos.
The mutation of Yap1-binding domains in Amotl2a does not render it unstable. MIP of Z-stacks of pLLP in cldnb:gfp embryos with the indicated genotype (A–D). (E) Corresponding boxplot comparing pLLP cell counts. (F) Western blot showing the stability of wild-type Amotl2a, Amotl2aFL (lane2) and Amotl2a forms carrying both PPEA-LATA (lane3) and single PPEA and LATA (lanes 4 and 5), respectively. α-tubulin staining shows equal protein loading (Figure 6—source data 4).