ZFIN Image: Masuda et al., 2016, Fig. S2

Image

Figure Caption

Fig. S2

Subcellular localization of ES1 in zebrafish cones. (a) Apparent heterogeneity of immunoreactivities in cone ellipsoid. High magnification views of double immunostainings of cone ellipsoids with TOM20/ES1 antibodies (upper panels) and TOM20/mAAT antibodies (lower panels). TOM20 antibody was used as a mitochondrial outer membrane marker to detect the outline of individual mitochondria. Both ES1- and mAATimmunopositive signals were weaker at apical (upward direction) and central regions of the cone ellipsoids (right panels). In the same regions, TOM20 antibody depicted clear round patches >2 µm in diameter (middle panels), indicating the presence of mega-mitochondria. (b) Subcellular fractionation analysis for ES1. Immunoblotting analysis with anti-ES1 antibodies was performed using fractionated cells from retinas. Anti-alpha-tubulin and TOM20 antibodies were used as a marker for soluble fraction and membrane fraction, respectively. S: soluble fraction, M: membrane fraction.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.