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Fig. 7
lnx2a-MO injection results in impaired cell proliferation in the ventral pancreas. (A) Tg(ptf1a:EGFP) embryos were incubated in 10 mM BrdU from 46 to 47 hpf, fixed, stained with anti-BrdU and anti-Numb antibodies, and analyzed by confocal microscopy. For clarity, the same plane of the BrdU channel is shown separately in addition to the merged images. (Scale bar: 10 µm.) (B) The average number of ptf1:EGFP+, Numb+ and ptf1:EGFP+/BrdU+ double-positive cells in the pancreas of 10 uninjected and lnx2a-MO–injected embryos are shown (***P < 0.001).
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Full text @ Proc. Natl. Acad. Sci. USA