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Fig. 6
lnx2a-MO injection causes a reduction of Notch-responsive cells in the pancreas. (A) Tg(Tp1:H2BmCherry); Tg(Tp1:VenusPest) embryos were injected with lnx2a-MO (A2), numb-MO (A3), or both (A4). At 48 hpf, embryos were stained with anti-Numb antibody and analyzed by confocal microscopy; Z projections are shown. (Scale bar: 10 µm.) (B) Total number of Notch-OFF (Tp1:H2BmCherry+) and Notch-ON (Tp1:H2BmCherry+ and Tp1:VenusPest+) cells, as well as Numb-positive cells, were counted in all Z sections of four embryos for each category. **P < 0.005; ***P < 0.002.
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