ZFIN Image: Bojarczuk et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Quantification of macrophage behavior in response to Cryptococcus during infection.(a) Maximum intensity z-projection of example image data from high content imaging (see Supplementary Fig. S1) of Tg(fms:Gal4.VP16)i186 ; Tg(UAS:nfsB.mCherry)i149 zebrafish, with mCherry labeled macrophages (magenta), infected with 148 cells of C. neoformans strain H99GFP (green), at 2 hours post infection. (b-d) Areas boxed in (a) enlarged and reconstructed in three-dimensions. Arrowheads indicate intracellular cryptococci. Image pairs represent different views of same volume with arrows indicating z-axis direction. Image grid is 20 µm. Images are representative of a total of 120 infections from n = 3 repeats (40 infections per independent repeat group).

Acknowledgments

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