ZFIN Image: Samuel et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Optic disc coloboma and optic nerve malformations in six3a;six3b double mutant embryos.

(A) Schematic presentation of WT Six3a and Six3a^L183S proteins. Amino acid numbers are depicted and location of L183S in the homeodomain is marked with an arrow. HD, homeodomain. (B) Chromatograms showing the sequence difference between WT (left) and mutant (right) six3a. (C-F) Lateral views (C,D) and ventral views (E,F) of 3 dpf live normal (C,E) and Six3-deficient (D,F) embryos. Arrows in (C,D) point at lenses, arrowheads in F point at the medial, incomplete RPE. (G-J) Low (G,H) and higher (I,J) magnifications of histological transverse sections through forebrains of normal (G,I) and Six3-deficient (H,J) 5 dpf embryos. “R” in (H) marks ectopic retinal tissue of one eye. Arrowhead and arrows in I point at the optic nerve inside and outside the eye, respectively, which cannot be clearly seen in the double mutant; asterisks mark location of the optic chiasm; bracket in J marks an apparently defasciculated optic nerve. (C,D,G-J) dorsal is up, (C-F) anterior is to the left. Scale bars are 50 µm.

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Phenotype details

Acknowledgments

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