ZFIN Image: Tang et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Visualizing the emergence of clonal dominance in Myc-induced T-ALL.

(a) Donor animals engrafted with monoclonal T-ALL arising in the CG1 background. (b,c) Monoclonal T-ALLs were implanted into the syngeneic CG1 strain fish and assessed for LPC frequency by limiting dilution cell transplantation (b) or latency of regrowth (c). P-values are noted within each panel. Not significant (NS). (d,e) Confocal imaging of engrafted rag2^E450fs (casper) fish at 10 d.p.t. (d) and 23 d.p.t. (e). White arrow denotes site of injection, and the location for confocal imaging. (f) Relative proportions of each fluorescent clone contained within leukaemias from individual engrafted animals (n=16 animals). Imaging was completed on the same animals at 10-13 d.p.t. and 22-24 d.p.t. The ZsYellow+ clone dominates leukaemia regrowth in animals #1-3, whereas mCherry+ dominates in animals #13-16. Scale bars equal 5 mm in whole animal images and 50 µm in confocal images.

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Acknowledgments

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