ZFIN Image: Sharif et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Overview of the experimental procedure: (A) time line of the experimental procedures experiment 1. Analyses 2 and 3 were performed at 4 dpf while analyses 1 and 3 were done at 5 dpf. (B) Time line of experiment 2 (C) caudal fin amputation, amputation plane (boxed area). (D) Morphological analysis. Top (normal tail), middle (Tail cut), bottom (area ‘r’ is regenerated caudal fin at 5 dpf). Analysis 1 includes the measurement of regenerated area caudal fin. Analysis 2 includes necrosis, cell proliferation, and apoptosis assays. Analysis 3 includes RT-qPCR analysis for expression of GR, MR, hoxd11, msxb, Wnt3a, and RARγ mRNA′s, quantitative analysis of L-plastin-positive macrophages, and qualitative assessment of neutrophil and macrophage migration in lysC: DsRED2 transgenic embryos. Abbreviations: DEX, dexamethasone; GR, glucocorticoid receptor; MR, mineralocorticoid receptor; nc, notochord; cf, caudal fin; N/A, not applicable; dpf: days post fertilization.

Acknowledgments

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