ZFIN Image: Shen et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Related to Figure 2. rraga mRNA is maternally expressed and widely distributed in the embryo.

(A) Time course of rraga mRNA expression analyzed by RTPCR of whole-animal lysates prepared at the indicated stages before (black) and after (red) the maternal-zygotic transition. RNA for rraga is detected before the zygotic transcription is active, in contrast to zygotically expressed control genes twist2 and plp1a. (B) Representative image of a 2 dpf embryo stained by in situ hybridization with antisense and sense probes for the rraga transcript. The antisense probe detected widespread expression, whereas the control sense probe showed background staining.

Acknowledgments

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