ZFIN Image: Ye et al., 2016, Fig. S3

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Figure Caption

Fig. S3

Pancreatic β cell mass normalizes to wild type levels at 96 hpf after continual induction of HOT:dnIRS2. (A-B′′) Merged and single channel confocal projections of 96 hpf HOT:dnIRS2 Cre- control (A-A′′) and HOT:dnIRS2 (B-B′′) islets after heat shock at 10 hpf, 24 hpf, 28 hpf, 32 hpf, 36 hpf, 48 hpf, 56 hpf ,72 hpf and 80 hpf. Ubiquitous induction of dnIRS2- GFP occurs only in the Cre+ embryos. (C) Quantification of β cells in 96 hpf HOT:dnIRS2 Cre-control (n=12) and HOT:dnIRS2 (n=10) islets; β cell mass is indistinguishable between the two conditions. Student’s t-test was used to determine significance in C.

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.