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Fig. S10
Insulin knockdown induces ectopic Pdx1 expression in ventral endoderm. Merged and single channel confocal images of 24 hpf control (A-B′) and insaMO-injected (C-D′) Tg(ins:CFP-NTR) islets immunostained for CFP (β cells, green), Pdx1 (red), and DNA (blue). (A) Confocal projection and (B-B′) higher power magnification confocal plane of control islet. (C) Confocal projection and (D-D′) higher power magnification confocal plane of insaMO-injected islet. Note the formation of β cells from Pdx1+ ventral endoderm (ve) in insaMO-injected embryos (yellow arrow). Abbreviation: pi, principal islet.
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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.