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Fig. S8
Serum deprivation demonstrates that TRCs extravasate more effectively than control melanoma cells in vivo. (a) TRCs and Cont after serum deprivation (0.1% serum containing medium cultured tumor cells for 24 hrs before injection) were injected into the pericardium of 48 hpf embryos respectively. Images in each panel show vessel penetration of Cont (left panels) or TRCs (right panels) at 1, 12, and 24 hpi respectively. Dashed white lines mark the tumor extravasation areas (i.e., various sizes of micrometastases) from vessels to surrounding tissues. Scale bars, 100 µm. (b) Quantification of extravasated tumor area relative to the total tumor area at different time points: 1, 12, and 24 hpi. Color code: Zebrafish blood vessels are green, and mouse tumor cells are red. TRCs exhibit higher penetration rates than Cont. Mean± s.e.m.; n>6 fish per group; ≥3 independent experiments. *p< 0.05.