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Fig. 8
Polarized migration is disrupted in Map1b-depleted embryos. a Selected frames from time-lapse imaging of control (uninjected) and map1b-depleted mGFP-expressing cells in the neural plate. The white dotted line indicates the dorsal midline, when visible in the imaging field. Time elapsed (minutes) is indicated in the upper right corner. Red asterisks indicate individual cells identified in multiple frames. Scale bar: 10 µm. b Representative traces of control and map1b-depleted cells traced over time. Traces corresponding to time 0 (t0 min, green) are to the right and traces of older cells (t56 min and higher, yellow) are to the left. c Plot of the average distribution of membrane protrusions in representative mGFP- labeled control and map1b-depleted cells at the neural plate stage. The red dotted line represents the position of the dorsal midline.