Fig. 2
Light-Controlled Shuttling of Protein between Cytoplasm and Membrane In Vivo
(A) Sequential images of a horizontal confocal slice through the developing neuroepithelium of a 15-somite embryo labeled with PHYB-MCherry-CAAX and PIF6-EGFP. The embryo was illuminated with alternating 5-min exposures to 650- and 750 nm light. PIF6-EGFP was recruited to the membrane after 650 nm illumination and released from the membrane into the cytoplasm after 750 nm illumination.
(B) (i) Relative PIF6-EGFP intensity after alternating 650- and 750 nm illumination, normalized to mean 750 nm levels. Error bars denote SEM. (ii) Illustration of PIF6-EGFP intensity sampling areas in membrane and cytoplasm. (iii) Raw image of (ii).
See also related Figure S2.
Reprinted from Developmental Cell, 36, Buckley, C.E., Moore, R.E., Reade, A., Goldberg, A.R., Weiner, O.D., Clarke, J.D., Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo, 117-126, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell