ZFIN Image: Ulrich et al., 2016, Fig. S2

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Fig. S2

nft^y72/sdp^w15 trans-heterozygotes and both nft^y72 and sdp^w15 homozygotes have identical CtA and DRG deficits. (A-H) Confocal lateral images of the Hb (A-D) and trunk (E-H) of 72 hpf embryos. Anterior, left; dorsal, up. Vasculature (white labels), red (Tg(kdrl:RFP)^s896); DRG neurons, green (in yellow circles, Tg(neurog1:eGFP)^w61). Scale bars: 100 µm. (I) Quantification of the abundance of missing, incomplete and complete Parachordal Chains (PAC) at 72 hpf in nft^y72 (reck^y72) and sdp^w15 (Df(Chr24:reck)^w15) mutants and their phenotypically-WT siblings. A complete PAC spans the full distance between an ipsilateral Se vessel pair. PAC percentages were thus calculated in reference to the total number of Se vessel pairs scored. Asterisks, significant differences (p < 0.05) between genotypes (Student′s t-test); n.s., no significant. n=10 embryos per genotype or phenotype.

Acknowledgments

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