ZFIN Image: Yokota et al., 2015, Fig. 9 S3

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Figure Caption

Fig. 9 S3

Vegfr3 is partially involved in increases in oscillating cells in dll4 morphants.

(A) The number of Ca²⁺-oscillating ECs at each somite boundary of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos injected with both dll4 and vegfr3 MOs was quantified at somite boundaries (total 38) at 24–27 ss as in Figure 4A. (B) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos injected with both dll4 and vegfr3 MOs during tip cell budding (24 ss). Green and blue arrowheads indicate two neighboring Ca²⁺-oscillating ECs, both of which are budding from the DA. (C) Fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from B indicated by arrowheads at the left panel are shown as a graph. Scale bar, 10 mm in B.

Acknowledgments

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