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Fig. 7 S1
Ca2+ responses after stalk cell budding from the DA are dependent upon Vegfr.
(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos treated with ki8751 after stalk cells completely migrate out of the DA as in Figure 7A. The embryos were treated from 28 ss with ki8751 and time-lapse imaged at 29 ss. Green and orange arrowheads indicate tip and stalk cells, respectively. (B) The fluorescence changes in GCaMP7a (ΔF/F0) of individual ECs from A indicated by arrowheads at the left panel are shown as a graph. Scale bar, 10 µm in A.
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