ZFIN Image: Yokota et al., 2015, Fig. 4 S3

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Figure Caption

Fig. 4 S3

PlexinD1 is necessary to confine Ca²⁺-oscillating sprouts in the vicinity of somite boundaries.

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos during tip cell budding injected with plxnD1 MO (18 ss). Arrowheads indicate Ca²⁺-oscillating ECs budding from the DA. Yellow dashed lines indicate positions of somite boundaries. Note that plxnD1 morphants display ectopic EC sprouts that exhibit Ca²⁺ oscillations. (B) Fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs indicated by arrowheads in A are shown as a graph. Scale bar, 10 mm in A.

Acknowledgments

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