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Fig. 4 S1
ECs close to somite boundaries have potential to sprout.
Light-sheet z-stack fluorescence images of Tg(fli1:Gal4FF);(UAS:GCaMP7a) (lower) and corresponding 2D-slice bright-field (BF) images at the level of somite boundary (upper) just after vessel sprouting (22 ss). Yellow arrowheads indicate somite boundaries. Yellow dashed lines indicate positions of somite boundaries. White arrowheads indicate Ca2+-oscillating cells, either of which extends protrusions dorsally. Note that double (left) or single (right) Ca2+-oscillating cells are located at somite boundary. Scale bar, 10 µm.
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