ZFIN Image: Yokota et al., 2015, Fig. 1 S1

Image

Figure Caption

Fig. 1 S1

GCaMP7a works as a Ca²⁺ indicator in endothelial cells (ECs).

(A) HUVECs transfected with GCaMP7a expression plasmids were treated with DMSO (upper) or ionomycin (lower). GCaMP7a images before (-) and after the treatment (40 s) are shown. (B) Confocal stack fluorescence images of Tg(fli1:Gal4FF);(UAS:GCaMP7a) embryos at 26 ss treated with DMSO (upper) or 25 µM ionomycin (lower). GCaMP7a images before (-) and after the treatment (30 min) are shown. (C) Fluorescence images of HUVECs transfected with GCaMP7a expression plasmids pretreated with DMSO or 25 µM BAPTA-AM for 30 min and treated with 50 ng/ml VEGF-A before (-) and after the treatment (100 s). Note that enhancement of GCaMP7a fluorescence by VEGF-A is blocked by pretreatment with BAPTA-AM. Scale bars, 10 µm in A-C.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife