Fig. 2
The SIVP vascular pattern is variable among embryos and develops superficial and deep vessel baskets. Depth-coded confocal Z-projections of SIVP development in two individual embryos (A–C′ and D–F′) from 2 to 4 dpf show a similar organization, but small differences in pattern. (A, D) At 2 dpf, the SIVP appears as a single basket on the surface of the yolk. (B, E) At 3 dpf a deeper second vascular basket is visible (the border of the inner basket is indicated by white arrowheads). (C, F) This inner basket becomes more evident at 4 dpf. Some vessels connect both inner and outer vessels (red arrowheads). (A′–F′) Enlargements of images in A–F. (G) Schematics from WT 1 embryo images A–C. Arrowheads indicate the boarder of the inner basket. Scale bars represent 100 µm.
Reprinted from Developmental Biology, 409(1), Goi, M., Childs, S.J., Patterning Mechanisms of the Sub-Intestinal Venous Plexus in Zebrafish, 114-28, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.