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Fig. S2
Anatomical distribution of the gastrointestinal organs and their vasculature (A-C) Confocal images of Tg(gut:GFP;fli1:dsRed) (A,B) and Tg(-1.0ins:EGFP; fli1:dsRed) (C) embryos at 3-4 dpf. (A′-C′) Explanatory diagrams of the corresponding confocal images, depicting the relative position of veins (grey), arteries (black), and organs (green). (A′′-C′′) Digital sections (10-15µm) performed at the approximate position indicated by the dashed line. (A-A′′) Hepatic vessels (HV) surround and penetrate the liver (L) by 4 dpf, and connect to the hepatic portal vein (HPV) and aPCV. (B-B′′) The SIV, SIA and ICVs vascularize the intestinal bulb (IB). (C-C′′) The first right ICV interconnects the right SIV with the SIA, and gives rise to vessels penetrating the pancreatic anlage. aPCV, anterior PCV; HPV, hepatic portal vein; HV, hepatic vessels; ICV, interconnecting vessels; SIV, subintestinal vein; IB, intestinal bulb; P, pancreas; PV, pancreatic vessels. Scale bars: 50µm.